Composite

Part:BBa_J72184

Designed by: Josh Kittleson   Group: Anderson Lab   (2012-09-17)

RepA Expression Cassette


Expresses RepA, the replication initiator protein for the origin of plasmid ColE2-P9.

Composition: BBa_J72073.BBa_J72160.BBa_J72049

Referred to internally as jtk3026

This part is in BBb Format. It is flanked by BglII and BamHI sites instead of XbaI and SpeI. More information about the BBb Format is available at:

[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None